Routinely evaluated by immunoprecipitation. Comes in a package of 5 immunoprecipitations.Ĭatch and Release® v2.0 should not be used with proteins containing a 6-His tag. Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)Ĭatch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types.Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209).Catch and Release® Wash Buffer, 10X (Cat.# 20-210). ![]() Please read the enclosed product manual before use. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. IP using human IgG1-4 should be suitable. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. Reversible: simple elution of antigen:antibody complex in either native or denatured form Reproducible: eliminates aspiration steps that typically lead to diminished/lost sampleĬonvenient: spin-column format dramatically simplifies sample handling ![]() Key Specifications Table Species ReactivityĬatch and Release® v2.0 Reversible Immunoprecipitation System
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